Soybean cultivar S06-98181-G01-35167

ABSTRACT

The present invention is in the field of soybean variety S06-98181-G01-35167 breeding and development. The present invention particularly relates to the soybean variety S06-98181-G01-35167 and its progeny, and methods of making S06-98181-G01-35167.

THE FIELD OF THE INVENTION

The present invention is in the field of soybean varietyS06-98181-G01-35167 breeding and development. The present inventionparticularly relates to the soybean variety S06-98181-G01-35167 and itsprogeny, and methods of making.

BACKGROUND OF THE INVENTION

Soybean Glycine max (L) is an important oil seed crop and a valuablefield crop. However, it began as a wild plant. This plant and a numberof other plants have been developed into valuable agricultural cropsthrough years of breeding and development. The pace of the developmentof soybeans, into an animal foodstuff and as an oil seed hasdramatically increased in the last one hundred years. Planned programsof soybean breeding have increased the growth, yield and environmentalhardiness of the soybean germplasm.

Due to the sexual reproduction traits of the soybean, the plant isbasically self-pollinating. A self-pollinating plant permits pollen fromone flower to be transferred to the same or another flower of the sameplant. Cross-pollination occurs when the flower is pollinated withpollen from a different plant; however, soybean cross-pollination is arare occurrence in nature.

Thus the growth and development of new soybean germplasm requiresintervention by the breeder into the pollination of the soybean. Thebreeders' methods of intervening in the pollination depend on the typeof trait that is being bred. Soybeans are developed for a number ofdifferent types of traits morphological (form and structure),phenotypical, or for traits like growth, day length, temperaturerequirements, initiation date of floral or reproductive development,fatty acid contents, insect resistance, herbicide resistance and yield.The genetic complexity of the trait often drives the selection of thebreeding method.

Due to the number of genes within each chromosome, millions of geneticcombinations exist in the breeders' experimental soybean material. Thisgenetic diversity is so vast that a breeder cannot produce the same twocultivars twice using the exact same starting parental material. Thus,developing a single variety of useful commercial soybean germplasm ishighly unpredictable, and requires intensive research and development.

The development of new soybeans comes through breeding techniques, suchas: recurrent selection, mass selections, backcrossing, single seeddescent and multiple seed procedure. Additionally, markers' assistedbreeding allows more accurate movement of desired alleles or evenspecific genes or sections of chromosomes to be moved within thegermplasm that the breeder is developing. RFLP, RAPD, AFLP, SSR, SNP,SCAR, isozymes, are all forms of markers that can be employed inbreeding soybeans or in moving traits into soybean germplasm. Otherbreeding methods are known and are described in various soybeantextbooks.

When a soybean variety is being employed to develop a new soybeanvariety or an improved variety the selection methods includebackcrossing, pedigree breeding, recurrent selection, modified selectionand mass selection. The efficiency of the breeding procedure along withthe goal of the breeding is the factors for determining which selectiontechniques are employed. A breeder continuous evaluates the success ofthe breeding program and therefore the efficiency of any breedingprocedure. The success is usually measured by yield increase, commercialappeal and environmental adaptability of the developed germplasm.

The development of new soybean cultivars most often requires thedevelopment of hybrid crosses (some exceptions being initial developmentof mutants directly through the use of the mutating agent, certainmaterials introgressed by markers, or transformants made directlythrough transformation methods) and the selection of progeny therefrom.Hybrids can be achieved by manual manipulation of the sexual organs ofthe soybean or by the use of male sterility systems. Breeders often tryto identify true hybrids by a readily identifiable trait or the visualdifferences between Inbred and hybrid material. These heterozygoushybrids are then selected and repeatedly selfed and reselected to formnew homozygous soybean lines.

Mass and recurrent selection can be used to improve populations. Severalparents are intercrossed and plants are selected based on selectedcharacteristics like superiority or excellent progeny. Outcrossing to anumber of different parents creates fairly heterozygous breedingpopulations.

Pedigree breeding is commonly used with two parents that possessfavorable, complementary traits. The parents are crossed to form a F1hybrid. The progeny of the F1 hybrid is selected and the best individualF2s are selected; this selection process is repeated in the F3 and F4generations. The inbreeding is carried forward and at F5-F7 the bestlines are selected and tested in the development stage for potentialusefulness in a selected geographic area.

In backcross breeding a genetic allele or loci is transferred into adesirable homozygous recurrent parent. The trait is in the donor parentand is tracked into the recurrent parent. The resultant plant is likethe recurrent parent with the new desired allele or loci.

The single-seed descent method involves use of a segregating plantpopulation for harvest of one seed per plant. Each seed sample isplanted and the next generation is formed. When the F2 lines areadvanced to F6 each plant will be derived from a different F2. Thepopulation will decline due to failure of some seeds, so not all F2plants will be represented in the progeny.

New varieties must be tested thoroughly to compare their developmentwith commercially available soybeans. This testing usually requires atleast two years and up to six years of comparisons with other commercialsoybeans. Varieties that lack the entire desirable package of traits canbe used as parents in new populations for further selection or aresimply discarded. The breeding and associated testing process is 8 to 12years' of work prior to development of a new variety. Thousands ofvarietal lines are produced but only a few lines are selected in eachstep of the process. Thus the breeding system is like a funnel withnumerous lines and selections in the first few years and fewer and fewerlines in the middle years until one line is selected for the finaldevelopment testing.

The selected line or variety will be evaluated for its growth,development and yield. These traits of a soybean are a result of thevariety's genetic potential interacting with its environment. Allvarieties have a maximum yield potential that is predetermined by itsgenetics. This hypothetical potential for yield is only obtained whenthe environmental conditions are perfect. Since prefect growthconditions do not exist, field experimentation is necessary to providethe environmental influence and to measure its effect on the developmentand yield of the soybean. The breeder attempts to select for goodsoybean yield potential under a number of different environmentalconditions.

Selecting for good soybean yield potential in different environmentalconditions is a process that requires planning based on the analysis ofdata in a number of seasons. Identification of the varieties carrying asuperior combination of traits, which will give consistent yieldpotential, is a complex science. The desirable genotypic traits in thevariety can often be masked by other plant traits, unusual weatherpatterns, diseases, and insect damage. One widely employed method ofidentifying a superior plant with such genotypic traits is to observeits performance relative to commercial and experimental plants inreplicated studies. These types of studies give more certainty to thegenetic potential and usefulness of the plant across a number ofenvironments.

In summary, the goal of the soybean plant breeder is to produce new andunique soybeans and progeny of the soybeans for farmers' commercial cropproduction. To accomplish this the plant breeder painstakingly crossestwo or more varieties or germplasm. Then the results of this cross arerepeatedly selfed or backcrossed to produce new genetic patterns. Neweravenues for producing new and unique genetic alleles into soybeansinclude introducing mutations or transgenes into the genetic material ofthe soybean are now in practice in the breeding industry. These geneticalleles can alter pest resistance such as insect resistance, nematoderesistance, herbicide resistance, or they can alter the plant's diseasetolerance, or its fatty acid compositions, the amount of oil produced,and/or the amino acid compositions of the soybean plant or its seed.

The traits a breeder selects for when developing new soybeans are drivenby the ultimate goal of the end user of the product. Thus if the goal ofthe end user is to resist a certain plant disease so overall more yieldis achieved, then the breeder drives the introduction of genetic allelesand their selection based on disease resistant levels shown by theplant. On the other hand, if the goal is to produce a specific oil, witha high level of oleic acid and a lower level of linoleic acid, then thebreeder may drive the selection of genetic alleles based on levels offatty acids in the seed and accept some lesser yield potentials or otherless desirable agronomic traits.

The new genetic alleles being introduced in to soybeans are widening thepotential uses and markets for the various products and by-products ofthe oil from the seed plants such as soybean. A major product extractedfrom soybeans is the oil in the seed. Soybean oil is employed in anumber of retail products such as cooking oil, baked goods, margarinesand the like. Another useful product is soybean meal, which is acomponent of many foods and animal feedstuffs.

SUMMARY OF THE INVENTION

One embodiment of the invention relates to seed of a soybean cultivardesignated S06-98181-G01-35167. The invention relates to the plant fromthe seed designated S06-98181-G01-35167, or the plant parts. Theinvention also encompasses a tissue culture of regenerable cells, cellsor protoplasts being from a tissue selected from the leaf, pollen,stomatal cell, embryo, meristematic cell, root, root tip, anther,flower, ovule, seed, stem, pod, petal and the cells thereof.

The invention in one aspect covers a soybean plant, or parts thereof,having all of the physiological and morphological characteristics of thesoybean plant.

Another aspect of this invention is the soybean plant seed or derivedprogeny which contains a transgene which provides herbicide resistance,insect resistance, resistance to disease, resistance to nematodes, malesterility, or which alters the oil profiles, the fatty acid profiles,the amino acids profiles or other nutritional qualities of the seed.

The present invention further covers a method for producing a soybeanseed with the steps of crossing at least two parent soybean plants andharvesting the hybrid soybean seed, wherein at least one parent soybeanplant is the present invention. In another aspect of the inventioncovers the hybrid soybean seed and the progeny soybean plant andresultant seed, or parts thereof from the hybrid seed or plant or itsprogeny.

In an additional aspect, the invention covers a method for producing asoybean progeny from the invention by crossing soybean lineS06-98181-G01-35167 with a second soybean plant to yield progeny soybeanseed and then growing progeny soybean seed.

Yet another aspect of the invention covers a method for a breedingprogram using plant breeding techniques which employ the soybean plantS06-98181-G01-35167 as plant breeding material and performing breedingby selection techniques, backcrossing, pedigree breeding, markerenhanced selection, mutation and transformation.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the Geographic Segment Chart—GSEGC shows the breakout forgrain yield at standard moisture for S06-98181-G01-35167 acrossgeographic locations.

FIG. 2 shows the Group Mean chart (GRP_MN=Group Mean) of Grain Yield atstandard moisture for S06-98181-G01-35167. This chart shows YieldStability−Win >5% of trial mean, Tie + or −5% of trial mean. Loses <5%of trial mean. The chart's vertical axis=yield of target variety, itshorizontal axis=location average yield. When the target variety line isabove the location average line this is desirable. The RSQ of the targetvariety shows a number. This number when it is closest to 1=yieldstability.

DETAILED DESCRIPTION

The following data is used to describe and enable the present soybeaninvention.

Trait Code Performance Trait Description VHNO Variety/Hybrid NumberYGSMN Grain Yield at Std MST (standard moisture) - YGSMN MRTYN MaturityDays from planting MRTYN HLDGR Harvest Lodging HLDGR PLHTN Plant Height(cm) PRR_R Phytophthora Root Rot To PRR_R IC_(——)R Iron ChlorosisIC_(——)R SDS_R Sudden Death Syndrome SDS_R SCL_R Sclerotinia White MoldSCL_R FELSR Frogeye Leaf Spot FELSR STR_R Shattering STR_R GLDGR GreenLodging GLDGR PLBRR Plant Branching PLBRR EMRGR Emergence EMRGR

Syngenta Soybean Data Collection Traits and Timing Order 1 = Seedling, 2= Vegetative, 3 = Reproductive to grain-fill, 4 = Maturity Opportunisticratings should only be collected if differences occur. At least onevariety has a rating of 5 or greater and there is a spread of 3 in theratings. Trait # Loc # Loc # Loc Order Group Code Description TimingComments Stage 4 Stage 5 Stage 6 Scale 1 HS RRG_R Presence of V2-V4, 4-7DAYS Home Loc's Home Loc's Home Loc's Roundup 1 = R, RR gene AFTERSPRAYING 5 = seg, 9 = susc. 1 HS RUR_R Roundup Tolerance R1-R2 HomeLoc's Home Loc's Home Loc's 1-9 scale, 1 = no yellowing Yellowing 9 isextreme yellowing 1 OTH EMRGR Emergence - stand VE-V3 NK EPA_R = 2 4 1to 9 (1 = best) establishment, uniformity V2-V6, 2005 on and vigor useEMRGR 2 DIS IC_(——)R Iron Deficiency August Internal Field MG00-2LMG00-4.9 MG00-4.9 1 to 9 (1 = best) Chlorosis Nursery 2 DIS ICFLR IronDeficiency Initial flash Initial Field MG00-2L MG00-4.9 MG00-4.9 1 to 9(1 = best) Chlorosis Yellow V2 June-July Nursery Flash 2 DIS ICR_R IronDeficiency 2-3 weeks after Internal Field MG00-2L MG00-4.9 MG00-4.9 1 to9 (1 = best) Chlorosis Recovery, ICFLR Nursery (Stunting and/orGreen-up) 2 DIS PRR_R Phytophthora Root Scheduled Internal Field St. JoeSt. Joe 1 to 9 (1 = best) Rot Tolerance in Nursery for field tolerancepond 2 NEM SCN_R SCN Field Rating June Internal Field Conesville,Conesville, Field = 1-9 (1 best) Nursery St. Joe St. Joe 3 AGR FL_CRFlower Color R1 Confirmed Numeric for EDC Home Loc's Home Loc's HomeLoc's W = White; P = Purple; Seg = Mix 3 AGR PLCNR Plant canopy AugustGrowth expressive Opportunistic Opportunistic 1 to 9, 1 = Slender,(~R5-R6) locations. 5 = Intermediate, 9 = Bush 3 DIS ARSTR Asian RustRating When differenes Opportunistic Opportunistic Opportunistic 1 to 9(1 = best) occur 3 DIS BSR_R Brown Stem Rot When differenesOpportunistic Opportunistic Opportunistic 1 to 9 (1 = best) occur 3 DISCR_(——)R Charcoal Rot When differenes Opportunistic OpportunisticOpportunistic 1 to 9 (1 = best) occur 3 DIS DPC_R Stem Canker (Northern)When differences Opportunistic Opportunistic Opportunistic 1 to 9 (1 =best) occur 3 DIS DPM_R Stem Canker (Southern) When differencesOpportunistic Opportunistic Opportunistic 1 to 9 (1 = best) occur 3 DISFELSR Frogeye Leaf Spot When differences Opportunistic OpportunisticOpportunistic 1 to 9 (1 = best) occur 3 DIS SCL_R Sclerotinia White Whendifferences Opportunistic Opportunistic Opportunistic 1 to 9 (1 = best)Mold occur 3 DIS SDS_R Sudden Death Syndrome When differencesOpportunistic Opportunistic Opportunistic 1 to 9 (1 = best) occur 3 PERFGLDGR Green lodging R5 to R6 Where differences OpportunisticOpportunistic Opportunistic 1 to 9 (1 best) 1 = occur All erect; 5 = 45degrees; 9 = flat 4 AGR MRTYD Maturity Date (MMDD) - 95% R8 Homelocations + ~1-2 ~5 ~10 MMDD (Sep. 10 = 0910) of plants in row shed 1-2off leaves & pods turned mature site/station color 4 AGR HC.CR HilumColor R8 Numeric for EDC Home Loc's Home Loc's Home Loc's G = Gray; BR =Brown; IB = Imperfect Black; Bl = Black; Y = Yellow; BF = Buff; IY =Imperfect Yellow; Seg = Mix 4 AGR PB_CR Pubescence Color R8-HarvestNumeric for EDC Home Loc's Home Loc's Home Loc's G = Gray; T = Tawny; LT= Lt. Tawny; Seg = Mix 4 AGR PD_CR Pod Color R8-Harvest Numeric for EDCHome Loc's Home Loc's Home Loc's T = Tan; B = Brown; Seg = Mix 4 AGRPLBRR Plant branching R8-Harvest Growth expressive Optional Home Loc'sHome Loc's 1 to 9, 1 = no branching; locations. 5 = average branching; 9= profuse branching 4 AGR PLHTN Plant Height (cm) Harvest Growthexpressive Optional Home Loc's Home Loc's Taken in cm locations. 4 DISGS_(——)R Green Stem Harvest Where differences OpportunisticOpportunistic Opportunistic 1 to 9 (1 = best) occur 4 PERF HLDGR HarvestLodging R8-Harvest Where differences Opportunistic OpportunisticOpportunistic 1 to 9 (1 best) 1 = All occur erect; 5 = 45 degrees; 9 =flat 4 PERF HVAPR Harvest Appearance Harvest Where differencesOpportunistic Opprotunistic Opportunistic 1 to 9, 1 = Excellent, occur 5= Avg, 9 = Poor 4 PERF STR_R Shattering Harvest Hill plot planter,Opportunistic + Opportunistic + 1 to 9 (1 = best) 2006? Bay and St. JoeBay and St. Joe Roundup is a trademark of Monsanto

Trait Definitions Opportunistic Ratings developed in YT. Opportunisticratings should only be collected if differences occur. At least onevariety has a rating of 5 or greater and there is a spread of 3 in theratings. (Scale 1-9, 1 = Best). Emergence (EMRGR) A rating of theuniform establishment and growth of seedlings. Taken from V1-V3, (Scale1-9). Maturity (MRTYD) The month and day (MMDD) when 95% of the mainstem pods in the plot have reached their mature color. Plant Height(PLHTN) The average measured plant height in cm. Branching (PLBRR)Rating of the number of branches and their relative importance to yield.Taken at growth expressive locations. (Scale: 1-9, 1 = stick, nobranching, 3 = 1 seed bearing branch, 5 = average branching or 2-3 seedbearing branches, 7 = 3-4 seed bearing branches, 9 = profuse branching).Green Lodging (GLDGR) Rating based on the average of plants leaning fromvertical in R5 to R6 stage (Scale 1-9, 1 to 9 (1 best) 1 = All erect; 5= 45 degrees; 9 = flat). Harvest Lodging (HLDGR) Rating based on theaverage of plants leaning from vertical at harvest (scale 1-9, 1 to 9 (1best) 1 = All erect; 5 = 45 degrees; 9 = flat). Shatter (STR_R) Ratingof pre-harvest loses based on amount of plants with open pods (Scale1-9). Iron Deficiency Chlorosis (IC_(——)R) final rating = average ofinitial Yellow Flash (ICFLR) and recovery (ICR_R) taken 2-3 weeks afterinitial yellow flash. (Scale 1-9, 1 = Best). LS means analysis unequalentries and reps between years. Soybean Cyst Nematode (CN_1R, CN_3R,CN_5R, CN14R) Greenhouse screen - 30 day sceen using infested soil.Rating Scale based upon female reproduction index on a susceptible checkset where <10% = R; <30% = MR; <60% = MS; >60% = S. In priority order,the races screened include: 3, 14, 1 & 5. Phytophthora Root Rot Fieldtolerance (PRR_R) or actual gene (RPS_T). Sudden Death Syndrome (SDS_R)based on leaf area affected, scale 1-9. Can be GH or field. Brown StemRot (BSR_R) Greenhouse pot - root dip or field rating of leaf symptoms.(Scale 1-9) Root Knot Nematode Arenaria (MA_R), Incognita (MI_R),Javanica (MJ_R)RKN). Scale 1-9. Stem Canker North (DPM_R) Southern(DPM_R). Scale 1-9. Sulfentrazone (SUL_R) Greenhouse nursery ratingdamage of multiple rates. Scale 1-9. Metributzin (MET_R) Greenhousenursery rating damage of multiple rates. Scale 1-9. Hypocotyl Elongation(HYP_R) A rating of a variety's hypocotyl extension after germinationwhen planted at a 5″ depth in sand and maintained a warm germinationenvironment for 10 days. (Scale 1 = Long, 5 = Intermediate, 9 = Short)

Trait Definitions

Hypocotyl Elongation (HYPO) A rating of a variety's hypocotyl extensionafter germination when planted at a 5″ depth in sand and maintained awarm germination environment for 10 days.

Seedling Establishment (EMG) A rating of the uniform establishment andgrowth of seedlings.

Maturity (MAT) The number of days after Aug. 31 when 95% of the mainstem pods in the plot have reached their mature color.

Peroxidase Activity (Perox)—seed protein peroxidase activity is definedas a chemical taxonomic technique to separate cultivars based on thepresence or absence of the peroxidase enzyme in the seed coat. Ratingsare POS=positive for peroxidase enzyme or NEG=negative for peroxidaseenzyme.

Plant Height (PLTHT) The average measured plant height in centimeters.

Branching (BRANCH or PLBRR) Rating of the number of branches and theirrelative importance to yield. This rating is taken at growth expressivelocations.

Green Lodging (GLODGE or GLDGR) Rating based on the average of plantsleaning from vertical in R5 to R6 stage.

Stem Lodging (LODGE) Rating based on the average of plants leaning fromvertical at harvest. Lodging score (1=completely upright, 9=completelyprostrate),

Shatter (SHAT or STR_R) Rating of pre-harvest loses based on amount ofplants with open pods.

Iron Deficiency Chlorosis (IDC or IC_R) A composite rating of YellowFlash, Green-up, and Stunting in HpH soil. A 1 rating is the mostpositive for resistance to the symptoms listed and 9 is the leastpositive.

Phytophthora Root Rot (PGR) or (PFT) Greenhouse pot—root dip method forPFT and hypodermic needle method for rating PGR.

Root Knot Nematode (RKN) Greenhouse screen—30 day screen using infestedsoil. Rating Scale based upon female reproduction index on a susceptiblecheck set where <10%=R; <30%=MR; <60%=MS; >60%=S.

Stem Canker (STC) Based on number of lesions, scale 1-9.

Sulfentrazone (SULF) Authority™ (commercial herbicide) Greenhousenursery rating damage of multiple rates.

Metributzin (MET) Greenhouse nursery rating damage of multiple rates.

Definitions of Staging of Development

The plant development staging system employed in the testing of thisinvention divides stages as vegetative (V) and reproductive (R). Thissystem accurately identifies the stages of any soybean plant. However,all plants in a given field will not be in the stage at the same time.Therefore, each specific V or R stage is defined as existing when 50% ormore of the plants in the field are in or beyond that stage.

The first two stages of V are designated a VE (emergence) and VC(cotyledon stage). Subdivisions of the V stages are then designatednumerically as V1, V2, V3 through V(n). The last V stage is designatedas V(n), where (n) represents the number for the last node stage of thespecific variety. The (n) will vary with variety and environment. Theeight subdivisions of the reproductive stages (R) states are alsodesignated numerically. R1=beginning bloom; R2=full bloom; R3=beginningpod; R4=full pod; R5=beginning seed; R6=full seed; R7=beginningmaturity; R8=full maturity.

BROWN STEM ROT (BSR)—This disease is caused by the fungus Phialophoragregata. The disease is a late-season, cool-temperature, soilbornefungus which in appropriate favorable weather can cause up to 30 percentyield losses in soybean fields. For purposes of these tests theinformation is gathered in a greenhouse with a plant in a pot then aroot dip procedure is employed.

SUDDEN DEATH SYNDROME (SDS or SDS_R)—This disease is caused byslow-growing strains of Fursarium solani that produce bluish pigments inculture. The disease is a mid to late season, soil borne disease thatoccurs in soybean fields with high yield potential. Yield losses may betotal or severe in infected fields. Sudden Death Syndrome (SDS) is basedon leaf area affected. The scale used for these tests is 1-5 or ifidentified as SDS_R the scale is 1-9.

SOYBEAN CYST NEMATODE—The Soybean Cyst Nematode (SCN) Heteroderaglycines, is a small plant-parasitic roundworm that attacks the roots ofsoybeans. Soybean Cyst Nematode (SCN) for purposes of these tests isdone as a greenhouse screen—30 day screen using infested soil. Therating scale is based upon female reproduction index on a susceptiblecheck set where <10%=R (RESISTANT); <30%=MR (MODERATELY RESISTANT);<60%=MS (MODERATELY SUSCEPTIBLE); >60%=S (SUSCEPTIBLE). In priorityorder, the screening races include: 3, 14, & 1.

MATURITY DATE. Plants are considered mature when 95% of the pods havereached their mature color. The number of days is either calculated fromAugust 31 or from the planting date. (MR#) wherein # equals days.

RELATIVE MATURITY GROUP (RM). Industry Standard for varieties groups,based day length or latitude. Long day length (northern areas in theNorthern Hemisphere) is classified as (Groups 000, 00, 0). Mid daylengths variety groups lie in the middle (Groups I-VI). Very short daylengths variety groups (southern areas in Northern Hemisphere) areclassified as (Groups VII, VIII, IX).

SEED YIELD (Bushels/Acre). The yield in bushels/acre is the actual yieldof the grain at harvest.

SHATTERING. The rate of pod dehiscence prior to harvest. Pod dehiscenceinvolves beans dropping out of the pods. Shatter (SHAT) for these teststhe rating of pre-harvest loses is based on amount of plants with openpods.

PLANT. Means the plant, the plant's cells, plant protoplasts, plantcells of tissue culture from which soybean plants can be regenerated,plant calli, plant clumps, and plant cells that are intact in plants orparts of the plants, such as pollen, nodes, roots, flowers, seeds, pods,leaves, stems, pod and the like.

The present invention is S06-98181-G01-35167, a Maturity Group I. Thissoybean is developed for use of the beans and its byproducts.S06-98181-G01-35167 has an area of best adaptation which occurs within:Southern Minnesota, Southeast South Dakota, Wisconsin, Northern Iowa,and Michigan. The invention does carry herbicide resistance.

The traits of the invention are listed below.

TRAITS Plant Characteristics RR ® Y STS ® N Flower Color P PubescenceColor T Pod Color B Hilum Color BF % Protein 13% mst. 33.0 % Oil @ 13%mst. 19.2 Seed Size/Lb Stem Termination  2.0 Plant Health Rps Gene SPhytopthora Root Rot Tolerance  3.7 SCN RACE 1 FI % SCN RACE 3 FI % SCNRACE 5 FI % SCN RACE 14 FI % Root Knot Nermatode - Incognita Root KnotNermatode - Arenaria Stem Canker (Southern) Stem Canker Tolerance(Southern) Rps gene indicates the specific gene for resistance but ifnone are indicated then none are known to be present Y = Yes, has trait.N = no does not contain trait RoundUp Ready ®, Roundup ® and Roundup ®Ultra are trademarks of Monsanto Company. STS ® is a trademark ofDuPont. Ratings are on a 1 to 9 scale with 1 being the best.

The present invention has a specific gene, the 1k, for resistance toPhytophthora root rot. This plant does show a good general fieldresistance to the disease.

The instant invention provides methods and composition relating toplants, seeds and derivatives of the soybean cultivarS06-98181-G01-35167. Soybean cultivar S06-98181-G01-35167 has superiorcharacteristics. The S06-98181-G01-35167 line has been selfed sufficientnumber of generations to provide a stable and uniform plant variety.

Cultivar S06-98181-G01-35167 shows no variants other than expected dueto environment or that normally would occur for almost anycharacteristic during the course of repeated sexual reproduction. Someof the criteria often used to select in various generations include:seed yield, emergence, appearance, disease tolerance, maturity, plantheight, and shattering data.

The inventor believes that S06-98181-G01-35167 is similar to thecomparison varieties shown in the last table. However, as shown in thetable, S06-98181-G01-35167 differs from these cultivars.

Direct comparisons were made between S06-98181-G01-35167 and the listedcommercial varieties. Traits measured included yield, maturity, lodging,plant height, plant diseases, and IDC. The results of the comparison arepresented in below. The number of environments in which the varietieswere compared is shown.

The present invention S06-98181-G01-35167 can carry genetic engineeredrecombinant genetic material to give improved traits or qualities to thesoybean. For example, but not limitation, the present invention cancarry, the glyphosate resistance gene for herbicide resistance as taughtin the Monsanto patents (WO92/00377, WO92/04449, U.S. Pat. No. 5,188,642and U.S. Pat. No. 5,312,910) or STS mutation for herbicide resistance.Additional traits carried in transgenes or mutation can be transferredinto the present invention. Some of these genes include genes that givedisease resistance to sclerotina such as the oxalate oxidase (Ox Ox)gene as taught in PCT/FR92/00195 Rhone Polunc and/or an OxalateDecarboxylase gene for disease resistance or genes designed to alter thesoybean oil within the seed such as desaturase, thioesterase genes(shown in EP0472722, U.S. Pat. No. 5,344,771) or genes designed to alterthe soybean's amino acid characteristics. This line can be crossed withanother soybean line that carries a gene that acts to provide herbicideresistance or alter the saturated and/or unsaturated fatty acid contentof the oil within the seed, or the amino acid profile of the seed.

The present invention S06-98181-G01-35167 is employed in a number ofplot repetitions to establish trait characteristics. The presentinvention is grown in a number of regions for testing and researchingthe invention in various environmental interactions.

Geographic Summary

The target variety yield is given as a percent of the trial average atall locations shown in FIG. 1 and each geographic segment West to Eastor Central (Cntrl) To South East where there are three or morelocations. The plots for these trials are 17.5 foot long plots plantedin 30-inch spaces. The plants in the plots are a combination ofexperimental material and commercial material. There are usually 36varieties and there are approximately 300 plants of each variety withtwo replications in 20-25 locations. The data in FIG. 1 is only chartedif there are at least 3 or more locations. N>=3

The present invention differ from the comparison commercial soybeanlines in that it is the mean of the group of soybeans (GRP_MN) that weretested and displayed in this geographic segment chart (GSEGC). When allof the regions that the testing data for the present invention areincluded the present invention is about 1.8% above the group mean.However, this invention works very well in the West where it is abovethe Group Mean by 2.8%. The data in the central region also shows verygood yield and moisture.

The present invention S06-98181-G01-35167 (identified herein as 35167)is employed in a trial for testing a number of environmentalinteractions. The results of the grain yield at standard moisture areshown in the chart as shown in FIG. 2. The present invention is avariety that will out yield most soybeans not only in a high yieldingenvironment, but also across extremely low yielding environments whencompared with the group mean. These tests allow the usefulness of theinvention to be shown in light of the environmental geneticinteractions.

Research Data 2005 Yield Performance Chart VHNO YGSMN MRTYN HLDGR PLHTNPRR_R IC_(——)R SDS_R SCL_R FELSR STR_R GLDGR PLBRR EMRGR 35167 61.6131.6 4.7 95.5 3.7 4.5 2.1 6.4 4.5 3.1 S19-R5 61.5 131.1 2.2 89.5 4.15.2 5.2 2.5 4.5 3.4 S17-P9 60.8 129.0 3.3 88.3 4.0 7.1 3.9 4.7 4.3 3.4Commercial 60.6 132.7 1.7 90.2 4.0 4.2 2.7 2.0 4.2 4.4 1 H-1852RR 60.3128.8 1.6 88.3 3.3 4.3 3.7 2.8 3.1 3.2 2012RR/N 59.6 133.5 3.4 91.4 3.06.3 2.9 4.7 4.5 3.7 1827RR/STS 59.0 130.9 3.0 86.5 2.8 3.3 2.5 4.8 4.12.7 1703RR 58.7 130.6 4.9 98.1 4.1 2.7 1.8 7.2 5.1 3.2 Commercial 58.3132.0 3.6 94.2 2.5 5.0 3.0 3.7 3.6 3.3 2 S17-A1 58.1 128.9 2.3 82.6 4.26.6 2.9 3.6 3.7 4.0 Commercial 56.1 128.5 2.6 85.4 3.9 3.4 2.0 5.0 3.74.7 3 Environ- 19 7 9 4 1 3 2 4 3 3 ments Mean 60.5 132.3 3.2 92.5 3.84.6 3.1 4.4 4.3 3.7 LSD (0.05) 2 1 1 6 1 2 1 1 1

Each of these lines has their own positive traits. Each of these linesis different from the present invention. The present invention has thehighest yield at standard moisture than each of the other 10 comparisonlines. The present invention shows more lodging then all of thecomparisons except one. The shatter rating is in the lower ⅓ of thegroup of tested lines and the ID-R rating is about average for thisgroup of lines. The yield and other data is a snapshot of each of theselines' results in the specific environment and will differ when otherenvironmental interactions are measured.

This invention also is directed to methods for producing a new soybeanplant by crossing a first parent plant with a second parent plantwherein the first or second parent plant is the present invention.Additionally, the present invention maybe used in the varietydevelopment process to derive progeny in a breeding population orcrossing. Further, both first and second parent plants can come from thesoybean line S06-98181-G01-35167. A variety of breeding methods can beselected depending on the mode of reproduction, the trait, and thecondition of the germplasm. Thus, any such methods using theS06-98181-G01-35167 are part of this invention: selfing, backcrosses,recurrent selection, mass selection and the like.

The scope of the present invention includes any use onS06-98181-G01-35167 of marker methods. Through the use of markers suchas SSRs, RFLP's, SNPs, Ests, AFLPs, gene primers, and the like toidentify genetic alleles which can be identified and breed with markerassistance into the present invention with little to no superfluousgermplasm being dragged into the present invention. This results information of the present invention plus for example, cyst nematoderesistance, brown stem rot resistance, aphid resistance, phytothoraresistance, IDC resistance, BT genes or male sterility genes orglyphosate tolerance genes or drought tolerance genes or alleles and thelike. Transgenes maybe directly introduced into cultivar using geneticengineering and transformation techniques well known in the art, some ofwhich are described above, or are originally introduced into a donor,parent using genetic engineering and transformation techniques, andusing the donor in a marker assisted trait conversion process, the traitis moved for example by backcrossing. A transgene typically comprises anucleotide sequence whose expression is responsible or contributes tothe trait, under the control of a promoter capable of directing theexpression of the nucleotide sequence at the desired time in the desiredtissue or part of the plant. Constitutive, tissue-specific or induciblepromoters all have different purposes and each could be employed. Thetransgene may also comprise other regulatory elements such as forexample translation enhancers or termination signals. The transgene maybe adapted to be transcribed and translated into a protein, or to encodeRNA in a sense or antisense orientation such that it is not translatedor only partially translated.

The scope of the present invention also includes any use onS06-98181-G01-35167 of transformation methods. Transformation methodsare means for integrating new genetic coding sequences (transgenes) intothe plant's genome by the incorporation of these sequences into a plantthrough man's assistance. Many dicots including soybeans can easily betransformed with Agrobacterium. Methods of introducing desiredrecombinant DNA molecule into plant tissue include the direct infectionor co-cultivation of plant cells with Agrobacterium tumefaciens, Horschet al., Science, 227:1229 (1985). Descriptions of Agrobacterium vectorsystems and methods are shown in Gruber, et al., “Vectors for PlantTransformation, in Methods in Plant Molecular Biology & Biotechnology”in Glich et al., (Eds. pp. 89-119, CRC Press, 1993). Transformed plantsobtained via protoplast transformation are also intended to be withinthe scope of this invention. The most common method of transformationafter the use of agrobacterium is referred to as gunning ormicroprojectile bombardment. This process has small gold-coatedparticles coated with DNA (including the transgene) shot into thetransformable material. Techniques for gunning DNA into cells, tissue,explants, meristems, callus, embryos, and the like are well known in theprior art. The DNA used for transformation of these plants clearly maybe circular, linear, and double or single stranded. Usually, the DNA isin the form of a plasmid. The plasmid usually contains regulatory and/ortargeting sequences which assists the expression of the gene in theplant. The methods of forming plasmids for transformation are known inthe art. Plasmid components can include such items as: leader sequences,transmit polypeptides, promoters, terminators, genes, introns, markergenes, etc. The structures of the gene orientations can be sense,antisense, partial antisense, or partial sense: multiple gene copies canbe used.

After the transformation of the plant material is complete, the nextstep is identifying the cells or material, which has been transformed.In some cases, a screenable marker is employed such as thebeta-glucuronidase gene of the uidA locus of E. coli. Then, thetransformed cells expressing the colored protein are selected for eitherregeneration or further use. In many cases, a selectable markeridentifies the transformed material. The putatively transformed materialis exposed to a toxic agent at varying concentrations. The cells nottransformed with the selectable marker, which provides resistance tothis toxic agent, die. Cells or tissues containing the resistantselectable marker generally proliferate. It has been noted that althoughselectable markers protect the cells from some of the toxic affects ofthe herbicide or antibiotic, the cells may still be slightly affected bythe toxic agent by having slower growth rates. If the transformedmaterial was cell lines then these lines are regenerated into plants.The cells' lines are treated to induce tissue differentiation. Methodsof regeneration of cellular are well known in the art. The plants fromthe transformation process or the plants resulting from a cross using atransformed line or the progeny of such plants are transgenic plantsthat carry the transgene.

DEPOSIT INFORMATION

Applicants have made a deposit of at least 2500 seeds of soybeancultivar S06-98181-G01-35167 with the American Type Culture Collection(ATCC), Manassas, Va. 20110 on Jul. 25, 2007 and designated PTA-8550.The seeds were tested on Aug. 22, 2007 and found to be viable. Access tothis deposit will be available during the pendency of the application tothe Commissioner for Patents and persons determined by the Commissionerto be entitled thereto upon request. Upon granting of a patent on anyclaims in the application, the Applicants will make the depositavailable to the public pursuant to 37 CFR §1.808. Additionally,Applicants will meet the requirements of 37 CFR §1.801-1.809, includingproviding an indication of the viability of the sample when the depositis made. The ATCC deposit will be maintained in that depository, whichis a public depository, for a period of 30 years, or 5 years after thelast request, or for the effective life of the patent, whichever islonger, and will be replaced if it becomes nonviable during that period.

Accordingly, the present invention has been described with some degreeof particularity directed to the preferred embodiment of the presentinvention. It should be appreciated, though that the present inventionis defined by the following claims construed in light of the prior artso that modifications or changes may be made to the preferred embodimentof the present invention without departing from the inventive conceptscontained herein.

1. A soybean seed designated S06-98181-G01-35167, a sample of said seeddeposited under ATCC Accession No. PTA—8550.
 2. A plant, or partsthereof, produced by growing the seed of claim
 1. 3. Pollen of the plantof claim
 2. 4. A soybean plant, or parts thereof, having all of thephysiological and morphological characteristics of the soybean plant ofclaim
 2. 5. A tissue culture of regenerable cells of the soybean plantof claim
 2. 6. The tissue culture according to claim 5, wherein thecells are obtained from the group consisting of leaf, pollen, embryo,meristematic cell, root, root tip, anther, stomatal cell, flower, seed,stem and pod.
 7. A soybean plant regenerated from the tissue culture ofclaim 6, having all of the morphological and physiologicalcharacteristics of soybean cultivar S06-98181-G01-35167.
 8. A method forproducing a soybean seed comprising crossing two soybean plants andharvesting the resultant soybean seed, wherein at least one soybeanplant is the soybean plant of claim
 2. 9. A method for producing ahybrid soybean seed comprising crossing the soybean plant according toclaim 2 with a second soybean plant and harvesting the resultant hybridsoybean seed.
 10. A method for producing a S06-98181-G01-35167-derivedsoybean plant, comprising: a) crossing soybean line S06-98181-G01-35167,a sample of said line deposited under ATCC Accession No. PTA—8550, witha second soybean plant to yield progeny soybean seed; and b) growingsaid progeny soybean seed to yield said S06-98181-G01-35167-derivedsoybean plant.
 11. The method of claim 8, wherein the second soybeanplant is transgenic.
 12. The method of claim 11 wherein the transgenicsoybean plant contains genetic material selected from the groupconsisting of herbicide resistance, nematode resistance, insectresistance, resistance to disease, and male sterility.
 13. The method ofclaim 12 wherein the resistance to disease is through an oxalate oxidaseencoding polynucleotide sequence or an oxalate decarboxylase encodingpolynucleotide sequence.